![]() ![]() ![]() ![]() Why can I get a positive pregnancy test then negative? Once hCG is successfully sandwiched, when you take a pregnancy test this triggers release of a dye-that’s the dark line on a pregnancy test that means you’re pregnant! The first line on a pregnancy test is a control test that simply makes sure the antibodies and dye are working. The first antibody gloms on to the alpha unit (“peanut butter”) and the second antibody gloms on to the beta unit (“the grape jelly”), which forms a chemical sandwich. To detect both parts of hCG, a pregnancy test loads the stick with two different antibodies-let’s call them “the bread slices”. Let’s call the alpha unit of hCG “the peanut butter” and the beta unit “the grape jelly”. HCG is a really big molecule that is made of two parts: the alpha part and the beta part. It sounds complicated, and the chemistry of it is, but if you’ve made a peanut butter and jelly sandwich before, then you’ll understand how this test works. This type of a test is a sandwich enzyme immunoassay. How does a home pregnancy test work?Ī home pregnancy test measures amount of hCG in your urine. To explain why this happens, this post will cover how a pregnancy test works, what the hook effect is, and what you can do. If you take a pregnancy test after about week five of pregnancy, you might experience a type of false negative called the hook effect. You’re sure that you’re pregnant because you recently had a positive pregnancy test, but now a few weeks later, you see a faint pregnancy test line or no line at all. False negative results can be stressful part of your initial steps in pregnancy. ![]()
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